Main functions
1. Promote cell division, mainly the division of cytoplasm;
2. Promote bud differentiation. In tissue culture, it interacts with auxin to control the differentiation and formation of roots and buds;
3. Promote the development of lateral buds, eliminate apical dominance, and thus lead to the formation of a large number of adventitious buds in tissue culture;
4. Delay leaf senescence, slow down the degradation rate of chlorophyll and proteins;
5. Break seed dormancy, replace light to meet the light requirement of seeds such as tobacco;
6. Induce parthenocarpy in certain fruits;
7. Promote the formation of bud initials: at the cut ends of leaves and in some mosses, it can promote the formation of bud initials;
8. Stimulate the formation of potato tubers.
It only contains the trans structure and has the same effect as zeatin, but with a stronger activity.
Its effect is very similar to that of anti-zeatin. It not only has the above-mentioned functions of zeatin, but also has the effect of activating gene expression and metabolic activity.
Usage Method
1. Promote the germination of callus (must be used in combination with auxin), concentration 1mg/L.
2. Promote fruit setting, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, spray on fruits 10, 25, and 40 days after flowering.
3. For leafy vegetables, spray at 201 mg/L to delay leaf yellowing.
In addition, treating some crop seeds can promote germination; treating at the seedling stage can promote growth.
1. Promote the germination of callus tissue (must be used in combination with auxin), at a concentration of 1 ppm;
2. Promote fruit setting, 100 ppm of cytokinin + 500 ppm of GA3 + 20 ppm of NAA, spray the fruits 10, 25, and 40 days after flowering;
3. Delay the yellowing of vegetable leaves, spray 20 ppm;
1. In plant tissue culture, the common concentration of anti-cytokinin nucleoside is 1 mg/mL or higher.
2. In plant growth regulation, the concentration of anti-cytokinin nucleoside is usually 1 ppm to 100 ppm, and the specific concentration depends on the specific application and plant species. For example, when promoting the germination of callus tissue, the concentration of anti-cytokinin nucleoside is 1 ppm, and it needs to be used in combination with auxin.
3. Dissolve the anti-cytokinin nucleoside powder thoroughly with 2-5 mL of 1 M NaOH (or 1 M acetic acid or 1 M KOH), then add double-distilled water or ultrapure water to prepare a storage solution of 1 mg/mL or higher concentration. Stir while adding water to ensure thorough mixing. The storage solution should be aliquoted and frozen stored to avoid repeated freeze-thawing. Dilute the storage solution with the culture medium to the required concentration, and prepare the working solution on the spot and use it immediately.
In conclusion, zeatin, abscisic acid and abscisic acid nucleotide each have their own characteristics in terms of structure, activity and functional applications.In conclusion, zeatin, abscisic acid and abscisic acid nucleotide each have their own characteristics in terms of structure, activity and functional applications. However, they all function as plant growth regulators and play a significant role in promoting plant growth and development.
Post time: Oct-22-2025